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The use of metalion‐supplemented buffers to enhance the resolution of peptides in capillary zone electrophoresis
Author(s) -
Mosher Richard A.
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110915
Subject(s) - capillary electrophoresis , electrophoresis , resolution (logic) , chemistry , histidine , chromatography , metal , analytical chemistry (journal) , ion , metal ions in aqueous solution , amino acid , biochemistry , organic chemistry , artificial intelligence , computer science
The potential of metal ion‐containing buffers to enhance the resolution of peptides in capillary zone electrophoresis was evaluted. The impact of adding Cu(II) and Zn(II) salts to electrophoresis buffers is shown to affect the migrational behavior of several dipeptides containing histidine. Interaction with a metal ion differentially decreases the electrophoretic mobilities of peptides which comigrate in the absence of metal ions, thus causing their separation. This effect is obtained at low pH where the large net charge on the samples yields short analysis times. The dependence of the resolution on Zn(II) concentration is presented for two different samples. The influence of the background buffer is discussed.