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Mathematical description of electrophoretic separation of weak acids by means of an H + pulse
Author(s) -
Gebauer Petr,
Deml Mirko,
Pospichal Jan,
Boček Petr
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110909
Subject(s) - pulse (music) , electrophoresis , separation (statistics) , sample (material) , biological system , analytical chemistry (journal) , chemistry , physics , chromatography , mathematics , optics , statistics , detector , biology
The paper gives a detailed theoretical description of the recently published principle of separation by a dynamic pulse in zone electrophoresis. The theory is illustrated by using a system in which weak acids are separated by migrating against an H + pulse. The mathematical description pertains to (i) the evolution and migration of the concentration profile of the H + pulse, and (ii) the migration of a sample substance (here a weak acid) against and through the pulse. As a result the migration (detection) times of the separated sample components are obtained. Based on the theory, a graphical treatment was developed which allows fast optimization of the separation conditions by observing (on the PC monitor) how the trajectories of the substances (drawn in a time vs . distance coordinate system) change with a change in the parameters of the pulse. Some of the results illustrate the possible advantages of using the dynamic pulse method in analytical practice.