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Capillary isotachophoresis with UV and tandem mass spectrometric detection for peptides and proteins
Author(s) -
Smith Richard D.,
Fields Steven M.,
Loo Joseph A.,
Barinaga Charles J.,
Udseth Harold R.,
Edmonts Charles G.
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110907
Subject(s) - isotachophoresis , chemistry , chromatography , tandem mass spectrometry , tandem , mass spectrometry , peptide , capillary action , biochemistry , materials science , electrode , electrolyte , composite material
The application of capillary isotachophoresis (CITP) and combined CITP‐mass spectrometry (MS) for peptides and proteins is demonstrated. Separation of simple peptide mixtures, as well as enzymatic digets of proteins, is also reported using CITP with UV detection. The potential utility of CITP for proteins is demonstrated. Initial studies of combined CITP‐MS of enzymatic digests is also demonstrated, showing the potential for rapid sequence determination.