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Calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis
Author(s) -
Paulus Aran,
Gassmann Ernst,
Field Matthew J.
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110906
Subject(s) - chromatography , capillary electrophoresis , polyacrylamide , chemistry , oligonucleotide , analytical chemistry (journal) , electrophoresis , polyacrylamide gel electrophoresis , calibration , resolution (logic) , capillary action , calibration curve , molecular mass , materials science , detection limit , dna , biochemistry , statistics , mathematics , artificial intelligence , computer science , polymer chemistry , composite material , enzyme
Polyacrylamide‐filled gel columns are used to separate oligonucleotide samples. For homopolymeric standard samples, plots of migration time versus molecular size are presented over a range of 30–160 bases. With 2.5–4 % T and 3.3 % C gels, good resolution over the examined mass range, with peak width at half height of 3 to 6 s, is obtained by applying electrical fields of 200–400 V/cm. The separation of heteropolymeric nucleotides by slab gel electrophoresis under routine conditions was compared with capillary gel electrophoresis. Using the same column and the same separation conditions, the plot of migration time versus base number is linear with and identical slope for three oligonucleotide samples which were examined, allowing a calibration of a gel‐filled capillary for molecular mass determination.