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Native bromoperoxidases do not bind to nitrocellulose: Use of DEAE‐cellulose as an alternative in blotting
Author(s) -
Jordan Peter,
Vilter Hans
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110814
Subject(s) - nitrocellulose , blot , cellulose , concanavalin a , collodion , polyacrylamide gel electrophoresis , chemistry , glycoprotein , chromatography , gel electrophoresis , biochemistry , enzyme , membrane , in vitro , gene
Bromoperoxidases were investigated by protein blotting after polyacrylamide gel electrophoresis, While the denatured proteins bound to nitrocellulose, the native enzymes did not. Instead, they could be transferred successfully to DEAE‐cellulose. Procedures for immunostainig and glycoprotein detection with concanavalin A on DEAE‐cellulose are described. The resultes indicate that binding of native proteins to nitrocellulose can not necessarily be assumed. DEAE‐cellulose is suitable both to investigate this phenomenon or as a substitute for nitrocellulose in blotting of native proteins.