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Synthesis of thiomorpholino buffers for isoelectric focusing in immobilized pH gradients
Author(s) -
Chiari Marcella,
Righetti Pier Giorgio,
Ferraboschi Patrizia,
Jain Tikam,
Shorr Robert
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110806
Subject(s) - morpholino , isoelectric focusing , acrylamide , chemistry , sulfur , chromatography , isoelectric point , combinatorial chemistry , organic chemistry , biochemistry , polymer , enzyme , zebrafish , copolymer , gene
The two commercially available Immobilines having a p K of 6.2 (2‐morpholino ethyl acrylamide) and 7.0 (3‐morpholinopropylacrylamide) have been modified and two new buffers have been synthesized: 2‐thiomorpholinoethylacrylamide, p K 6.6, and 3‐thiomorpholinopropyl acrylamide, p K 7.4. The replacement of an oxygen with a sulfur atom in the morpholino ring is thus seen to shift the p K values of these two bases by +0.4 pH units. In formulations in which the two new bases replaced the standard morpholino derivatives, identical pH profiles and protein patterns were obtained. The reason for this work was to try to close the gap between the p K 7.0 and 8.5 species and to provide the users of immobilized pH gradients with more buffers in the neutral pH region. The two new thiomorpholino derivatives are an important step in this direction.