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Zymographic screening of plant peroxidase isoenzymes oxidizing 4‐hydroxystilbenes
Author(s) -
Calderón Antonio A.,
Pedreño M. Angeles,
RosBarceló Alfonso,
Muñoz Romualdo
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110614
Subject(s) - peroxidase , chemistry , isoelectric focusing , oxidizing agent , hydrogen peroxide , isozyme , biochemistry , enzyme , organic chemistry
A zymographic assay is described for the detection of peroxidase isoenzymes oxidizing 4‐hydroxystilbene following isoelectric focusing. The assay is based on coupling intermediate products of the oxidation of 4‐hydroxystilbene with 4‐aminoantipyrine, with resultant formation of dye complexes. Control experiments in the absence of 4‐hydroxystilbene and hydrogen peroxide demonstrate the peroxidative nature of the 4‐hydroxystilbene‐dependent dye reaction.