Premium
Electrophoretic separation of nucleic acids: Evaluation by video and photographic densitometry
Author(s) -
Freeman Steven E.,
Larcom Lyndon L.,
Thompson Bryan D.
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110513
Subject(s) - densitometry , agarose , electrophoresis , agarose gel electrophoresis , nucleic acid , chromatography , dna , ultraviolet , chemistry , materials science , optics , physics , biochemistry , optoelectronics
The separation of DNA by gel electrophoresis provides a rapid method for determining size distributions of DNA in solution. Densitometric scanning of photographs of gels has been the standard method of analysis of agarose gels. However, analysis of photographs is complicated by the non‐linear response of photographic film. Charged‐coupled device video cameras have become popular for quantitative densitometry and we have used a charge‐coupled device camera to image agarose gels to quantitate DNA damage. We compare video and photographic densitometry for quantitation of ultraviolet radiation (UV)‐induced DNA damage and find that the two methods give equivalent results.