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Image analysis techniques for automatic evaluation of two‐dimensional electrophoresis
Author(s) -
Häder DonatP.,
Kauer Gerhard
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110510
Subject(s) - computer science , microcomputer , software , interpolation (computer graphics) , artificial intelligence , computer vision , spline interpolation , computer graphics (images) , representation (politics) , scanner , image (mathematics) , programming language , bilinear interpolation , telecommunications , chip , politics , political science , law
Techniques for automatic analysis of two‐dimensional electrophoresis gels by computer‐aided image analysis are described. Original gels or photographic films are scanned using a laser scanner and the files are transferred to a microcomputer. The program package first performs a compression and preevaluation of the files. Spot identification and quantification is performed by the chain code algorithm after appropriate zooming and cutting. Labeling facilitates spot identification and quantification in numerical and graphical (pseudocolor) representation on peripheral devices for camera ready output. Interpolation between measured basepoints is performed by cubic spline algorithms which are automatically switched on and off, depending on the need by the program. High speed analysis and graphic representation is achieved using fast Assembler language routines rather than high level languages. One‐dimensional gels can be analyzed using the same software. Spot matching between parallel two‐dimensional gels has not yet been implemented.