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The application of schlieren optics for detection of protein bands and other phenomena in polyacrylamide gel electrophoresis
Author(s) -
Takagi Toshio,
Kubota Hidehiro
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110504
Subject(s) - schlieren , electrophoresis , optics , gel electrophoresis of proteins , distortion (music) , polyacrylamide gel electrophoresis , materials science , chemistry , chromatography , physics , optoelectronics , amplifier , biochemistry , cmos , enzyme
A modernized schlieren optics system was applied to follow protein bands visually during polyacrylamide gel electrophoresis. This approach is suitable for quantitative measurement of electrophoresis as well as for detection of protein bands because of the absence of distortion of the electrophoretic pattern during the staining process. The optics are also suitable for the detection of various background phenomena which usually escape observation.