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Autoradiography using storage phosphor technology
Author(s) -
Johnston Richard F.,
Pickett Siobhan C.,
Barker David L.
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110503
Subject(s) - phosphor , materials science , resolution (logic) , nuclear medicine , chemistry , optoelectronics , medicine , computer science , artificial intelligence
We describe the application of photostimulable storage phosphor imaging plates to autoradiography of samples labeled with 32 P, 14 C and 35 S. Imaging plates can detect a 32 P‐labeled sample at an exposure level of 1 disintegration/mm 2 , and 14 C and 35 S at 25 disintegrations/mm 2 . Compared to X‐ray film, imaging plates offer increased sensitivity of 15‐ to 250‐fold for 32 P and 20‐ to 100‐fold for 14 C and 35 S. Resolution is in the 0.3 mm range and is therefore suitable for any gel or blot application.