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Deoxyribonuclease zymography adapted to the precast PhastGel electrophoresis media
Author(s) -
Strætkvern Knut O.,
Raae Arnt J.
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110416
Subject(s) - isoelectric focusing , microelectrophoresis , deoxyribonuclease , zymography , chromatography , polyacrylamide gel electrophoresis , agarose , polyacrylamide , gel electrophoresis , sodium dodecyl sulfate , chemistry , electrophoresis , molecular weight size marker , gel electrophoresis of proteins , biochemistry , enzyme , polymer chemistry
A set‐up for casting fluorescent indicator agarose gels on ultrathin polyacrylamide microelectrophoresis gel media (Pharmacia PhastGel media) is described. The zymogram system allows a rapid and sensitive detection of deoxyribonuclease in various gel media, following isoelectric focusing, native and sodium dodecyl sulfate‐polyacrylamide gel electrophoresis.