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Phenotyping of erythrocyte acid phosphatase and esterase D by high field strength isoelectric focusing on cellulose acetate membrane
Author(s) -
Kane Masateru,
Yamamoto Yoshio,
Yamada Mitsuko,
Fukunaga Tatsushige,
Tatsuno Yoahitsugu
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110409
Subject(s) - isoelectric focusing , acid phosphatase , esterase , chromatography , cellulose acetate , chemistry , membrane , cellulose , biochemistry , isoelectric point , enzyme
Phenotyping of erythrocyte acid phosphatase (EAP) and esterase D (ESD) by cellulose acetate membrane isoelectric focusing (CAM‐IEF) under a nonequilibrium condition is described. In an attempt to improve the method of CAM‐IEF, we shortened the electrode distance to provide a higher field strength at a given (low) voltage. Various carrier ampholytes for EAP typing and various chemical separators for ESD typing were also tested. Good separations were obtained after 30 min IEF for EAP typing and 25 min for ESD typing. When applied to blood stains and stored for various periods at room temperature, the stains up to 8 months old could still be phenotyped for EAP and those up to 4 weeks old for ESD. CAM‐IEF is suitable for routine forensic work of EAP and ESD phenotyping.