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Isotachophoretic zone formation of serum albumin in different free fluid electrophoresis instruments
Author(s) -
Thormann Wolfgang,
Firestone Millicent A.,
Sloan Jeffrey E.,
Long Terry D.,
Mosher Richard A.
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110405
Subject(s) - isotachophoresis , chromatography , chemistry , capillary electrophoresis , capillary action , electrophoresis , albumin , separator (oil production) , materials science , biochemistry , thermodynamics , electrolyte , physics , electrode , composite material
Abstract The isotachophoretic behavior of a model protein, serum albumin, was examined (i) by computer simulation, (ii) by capillary isotachophoresis in HPE 100 and Tachophor 2127, (iii) by continuous flow isotachophoresis in Elphor VaP 22 and the BIO‐STREAM Separator and (iv) by recycling isotachophoresis in an apparatus of our own design. Variations in monitored zone shapes can be explained by differences in engineering aspects and fluid stabilization principles of the instruments.