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Experimental and theoretical description of the isotachophoretic behavior of serum albumin
Author(s) -
Thormann Wolfgang,
Mosher Richard A.
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110404
Subject(s) - isotachophoresis , electrolyte , cationic polymerization , chemistry , plateau (mathematics) , albumin , chromatography , capillary action , serum albumin , thermodynamics , biochemistry , polymer chemistry , mathematical analysis , physics , mathematics , electrode
Abstract The isotachophoretic behavior of serum albumin is examined for three anionic and one cationic electrolyte systems by (i) computer simulation, (ii) capillary isotachophoresis (ITP) and (iii) continuous flow ITP. The theoretical relationship between pH of the leading electrolyte and the steady state protein plateau concentration is presented for one of the anionic systems. With leading ion concentrations of the order of 10 mm, experimental protein plateau concentrations of 1.3–2.3 % w/v are obtained. The computer predictions are approximately half these values.