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A two‐dimensional gel protein database of noncultured total normal human epidermal keratinocytes: Identification of proteins strongly up‐regulated in psoriatic epidermis
Author(s) -
Celis Julio E.,
Crüger Dorthe,
Kiil Jette,
Dejgaard Kurt,
Lauridsen Jette B.,
Ratz Gitte P.,
Basse Bodil,
Celis Ariana,
Rasmussen Hanne H.,
Bauw Guy,
Vandekerckhove Joel
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110308
Subject(s) - epidermis (zoology) , keratinocyte , isoelectric focusing , biology , gel electrophoresis , microbiology and biotechnology , human skin , two dimensional gel electrophoresis , database , biochemistry , in vitro , genetics , proteomics , gene , anatomy , enzyme , computer science
A two‐dimensional (2‐D) gel database of proteins from noncultured total normal human epidermal keratinocytes has been established. A total of 1449 [ 35 S]methionine labelled proteins (1112 isoelectric focusing, 337 nonequilibrium pH gradient electrophoresis) were resolved and recorded using computer assisted (PDQ‐SCAN and PDQUEST software) 2‐D gel electrophoresis. By matching the protein patterns of total keratinocytes and transformed human amnion cells (master database; Celis et al., Leukemia 1988, 2 , 561–602) as well as by 2‐D immunoblotting and microsequencing of keratinocyte proteins, it was possible to identify 72 polypeptides in the keratinocyte database. The database also includes data on polypeptides that are synthesized at a higher level by keratinocytes enriched in basal cells, and on six secreted proteins which are produced, albeit at a reduced rate, by normal keratinocytes and that are strongly up‐regulated in psoriatic epidermis (Celis et al., FEBS Letters , in press).