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Synergistic induction of polypeptides by tumor necrosis factor and interferon‐gamma in cells sensitive or resistant to tumor necrosis factor: Assessment by computer based analysis of two‐dimensional gels using the PDQUEST system
Author(s) -
Beresini Maureen H.,
Sugarman Barry J.,
Shepard H. Michael,
Epstein Lois B.
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110307
Subject(s) - tumor necrosis factor alpha , cell culture , interferon gamma , cytokine , biology , interferon , microbiology and biotechnology , tumor necrosis factor α , cell , immunology , biochemistry , genetics
Abstract Tumor necrosis factor (TNF) synergistically enhanced the antiproliferative activity of interferon‐gamma (IFN‐γ) in both TNF‐sensitive and TNF‐resistant variants of the cervical carcinoma line, ME‐180. TNF alone had no apparent effect on the levels of synthesis of individual proteins in either of these variant cell lines as assessed by computerized two‐dimensional gel analysis of cell lysates using the PDQUEST system. However, IFN‐γ enhanced the levels of 18 polypeptides and suppressed the levels of 10 polypeptides in both cell lines. When used in combination in both cell lines, TNF and IFN‐γ induced the synthesis of 10 polypeptides that were not induced by either agent alone. These synergistically induced polypeptides may be crucial to the mechanism of the synergistic antiproliferative action of TNF and IFN‐γ in ME‐180 cells.