IPGMAKER: A program for IBM‐compatible personal computers to create and test recipes for immobilized pH gradients | Zendy

Altland Klaus | Zendy

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IPGMAKER: A program for IBM‐compatible personal computers to create and test recipes for immobilized pH gradients

Author(s) -

Altland Klaus

Publication year - 1990

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150110207

Subject(s) - subroutine , computer science , video graphics array , graphics , coprocessor , personal computer , ibm , computational science , concentration gradient , chemistry , computer hardware , operating system , chromatography , software , nanotechnology , materials science

The program “IPGMAKER” is a computational aid for creating and testing recipes for near‐linear immobilized pH gradients. It was written for fast IBM personal computers (with a Type 80386 processor and 80387 coprocessor) and compatibles equipped with a VGA, EGA or Hercules (mono) graphics card. The program is limited to the use of up to 10 acids and/or bases, and to ranges spanning between pH 2 and 12. The resulting recipes are presented either as final concentrations in the 2 chambers of a mixing device for linear gradients or as volumes from 0.2 moles/L stock solutions adjusted to a user‐defined average buffering power. One of the subroutines determines the pH, gradient slope and buffering capacity at any location of the gradient and includes a facility to estimate the p I of proteins from the composition of their primary structure.

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