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Identification and quantification of apolipoprotein D in normal human urine
Author(s) -
Holmquist Leif
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110119
Subject(s) - apolipoprotein b , urine , molecular mass , chemistry , immunoelectrophoresis , chromatography , polyacrylamide gel electrophoresis , gel electrophoresis , sodium dodecyl sulfate , excretion , apolipoprotein e , biochemistry , antibody , medicine , biology , cholesterol , enzyme , immunology , disease
Apolipoprotein D has been identified in normal human urine, using sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, followed by immunoblotting with monospecific antibodies. Urinary apolipoprotein D appeared as a main 33 000 u protein together with a minor fraction corresponding to its partially deglycoslated species of lower molecular mass. No high molecular mass forms of apolipoprotein D naturally occurring in plasma could be detected. The apolipoprotein D mean ± SD concentration assayed with rocket immunoelectrophoresis, in urine samples from nine apparently healthy normal men, was 1.4 ± 1.0 mg/L (range: 0.2–3.0 mg/L). Among the plasma apolipoproteins, apolipoprotein D behaves uniquely as regards its excretion in urine; the other apolipoproteins belonging to the A, B, C and E groups, although of low molecular masses, are present, at most, in trace amounts in normal urine.