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Horizontal two‐dimensional electrophoresis of cerebrospinal fluid proteins with immobilized pH gradients in the first dimension
Author(s) -
Mattila Kari,
Pirttilä Tuula,
Frey Harry
Publication year - 1990
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150110118
Subject(s) - isoelectric focusing , cerebrospinal fluid , immobilized ph gradient , electrophoresis , chromatography , chemistry , two dimensional gel electrophoresis , cerebrospinal fluid proteins , dimension (graph theory) , isoelectric point , gel electrophoresis , pathology , biochemistry , proteomics , medicine , mathematics , enzyme , pure mathematics , gene
A horizontal two‐dimensional electrophoresis method with immobilized pH gradient isoelectric focusing supplemented with carrier ampholytes in the first dimension was applied to cerebrospinal fluid (CSF) proteins. About 300 protein spots could be detected on the silver‐stained two‐dimensional maps of CSF samples. This highresolution method is a tool worthy of consideration for the research of CSF proteins and disease‐specific changes in different neurological disorders.