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Step change of counterion – a new option in capillary zone electrophoresis
Author(s) -
Sudor Jan,
Stránský Zdeněk,
Pospíchal Jan,
Deml Mirko,
Boček Petr
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150101114
Subject(s) - capillary electrophoresis , electrolyte , matrix (chemical analysis) , counterion , ionic bonding , chemistry , electrophoresis , ionic strength , chromatography , analytical chemistry (journal) , ion , organic chemistry , aqueous solution , electrode
In contrast to common capillary zone electrophoresis, with a constant composition of the background electrolyte, a new method is described, based on a change of the ionic matrix composition during the migration of the sample components. The proposed method expands the scale of p K 's of analyzed compounds in a single run and affords new potential for optimizing the separation. The step change of the ionic matrix composition was achieved by displacing the primary matrix by the modified ionic matrix (electrolyte) which migrated against the movement of the sample components. The separation thus proceeded in the primary ionic matrix (electrolyte) while the detection was performed in the modified matrix with resultant shortening of the analysis time and convenient quantitation. A model mixture of five compounds, with a difference of four units in p K could be well resolved.