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Study of grasshopper meiosis‐associated proteins using two‐dimensional polyacrylamide gel electrophoresis
Author(s) -
Percy Melanie J.,
Fletcher Hugh L.
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150101016
Subject(s) - grasshopper , meiosis , polyacrylamide gel electrophoresis , gel electrophoresis , gel electrophoresis of proteins , biology , electrophoresis , genetics , chemistry , biochemistry , ecology , gene , enzyme
Premeiotic and meiotic whole testes from grasshoppers were compared for the presence of meiosis associated proteins using one‐ and two‐dimensional sodium dodecyl sulphate‐polyacrylamide gel electrophoresis. One‐dimensional sodium dodecyl sulphate‐polyacrylamide gels detected differences between premeiotic and meiotic samples but two‐dimensional gels gave more precise results. Isoelectric focusing revealed only one meiosis‐associated protein, while nonequilibrium pH gradient electrophoresis detected five more. It is not known whether these proteins relate to the nuclear aspects of meiosis, or associated cellular changes. These proteins have been electrophoretically purified and monoclonal antibodies are being prepared.