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Simultaneous phenotyping of erythrocyte acid phosphatase and esterase D by nonequilibrium agarose isoelectric focusing
Author(s) -
DimoSimonin Noëlle,
BrandtCasadevall Conxita,
Gujer HansRudolf
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150101011
Subject(s) - isoelectric focusing , agarose , esterase , acid phosphatase , chromatography , chemistry , isoelectric point , biochemistry , taurine , phosphatase , enzyme , amino acid
Nonequilibrium agarose isoelectric focusing in a pH 4.5–7 gradient with 3‐( N ‐morpholinopropanesulfonic) acid (MOPS) and taurine as chemical separators presents a fast, easy and reliable method for the simultaneous determination of the common erythrocyte acid phosphatase and esterase D phenotypes in hemolysates and dried bloodstains. The application of the method to experimental and casework bloodstains is described.