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The impact of boundary conditions in free flow field step electrophoresis
Author(s) -
Heydt Ariane,
Mosher Richard A.
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150101007
Subject(s) - membrane , electrolyte , electrophoresis , chemistry , stability (learning theory) , ion exchange , flow (mathematics) , chromatography , conductivity , dialysis , field (mathematics) , analytical chemistry (journal) , mechanics , ion , computer science , physics , biochemistry , electrode , mathematics , medicine , organic chemistry , machine learning , pure mathematics
The impact of the type of membrane used to separate the electrolyte compartments from the separation cell for continuous flow field step electrophoresis is examined using both experimental and computer simulation data. It is shown that the distinct transport characteristics of ion exchange membranes and dialysis membranes may have an effect on the stability of the pH and conductivity gradients created by the buffer system. These effects are interpreted using computer simulation data. The limitations of the model used to portray the transport characteristics of ion exchange membranes are discussed as are the experimental conditions which provide the greatest stability for field step electrophoresis.