Premium
Reactivities of immunoglobulin E and immunoglobulin G subclasses identified by isoelectric focusing‐immunoprint in allergic patients
Author(s) -
Becker WolfMeinhard
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100817
Subject(s) - isoelectric focusing , subclass , immunoglobulin e , antibody , isoelectric point , chemistry , allergen , immunology , chromatography , allergy , biology , biochemistry , enzyme
Probing of IgE or IgG subclass reactivities on single allergenic components in an extract is tedious and time‐consuming under native conditions. Isoelectric focusing immunoblotting combines a powerful, highly resolving native separatior method with the specificity and sensitivity of immunological test methods. This method is easy and quick to perform. The potential usefulness of this method is demonstrated by eight examples of a type I or type III allergy analyzing IgE and IgG subclass reactivities and their distribution.