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Cystic fibrosis proteins detected by electrophoretic techniques
Author(s) -
GuyCrotte Odette,
Barthe Catherine,
Figarella Catherine
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100816
Subject(s) - cystic fibrosis , electrophoresis , chemistry , chromatography , microbiology and biotechnology , computational biology , biology , genetics
For the detection of the cystic fibrosis protein (CFP) in serum of cystic fibrosis (CF) carriers, thin‐layer polyacrylamide gel isoelectric focusing proved inappropriate as diagnostic test, but was useful for screening fractions on purification of CFP by chromatofocusing on a Mono P column. On sodium dodecyl sulfate‐polyacrylamide gel electrophoresis an M r 12 000 protein (P 12) was found in most CFP‐positive sera, indicating good correlation between these two CF‐associated proteins. Detection of the P 12 protein by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis was well reproducible and less delicate than IEF. The technique was also used to purify P 12 from serum by two successive preparative electrophoresis steps in a 7.5–15 % gradient and 15 % homogeneous gel. The use of silver staining revealed that P 12 which was present in all sera of CF patients and carriers with variable intensities, was also present in trace amounts in normal sera.