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Electrophoretically silent hemoglobin mutants as revealed by isoelectric focusing in immobilized pH gradients
Author(s) -
Righetti Pier Giorgio,
Gianazza Elisabetta,
BianchiBosisio Adriana,
Wajcman Henri,
Cossu Gianfranco
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100811
Subject(s) - isoelectric focusing , immobilized ph gradient , hemoglobin , mutant , chromatography , isoelectric point , hemoglobin variants , capillary electrophoresis , chemistry , electrophoresis , amino acid , biochemistry , enzyme , gene
The applications of isoelectric focusing in immobilized pH gradients to the analysis of (i) human hemoglobin mutants, (ii) animal hemoglobin mutants (from cattle, sheep, dog and mouse), and (iii) tryptic digests of α and β chains, are discussed and evaluated. Immobilized pH gradients appear to be an excellent tool for screening of genetic polymorphism and for detecting “silent mutants”, i. e . those substitutions involving amino acids with nonionizable side chains. At present, not even capillary zone electrophoresis, claimed to have a resolving power equivalent to 1 million theoretical plates, has shown a resolution capability comparable to that of immobilized pH gradients, at least in the field of protein separation.