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Fast isoelectric focusing of milk proteins on small ultrathin polyacrylamide gels containing urea
Author(s) -
Moio Luigi,
Luccia Aldo Di,
Addeo Francesco
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100716
Subject(s) - isoelectric focusing , urea , polyacrylamide , chromatography , coomassie brilliant blue , chemistry , polyacrylamide gel electrophoresis , silver stain , isoelectric point , staining , biochemistry , biology , microbiology and biotechnology , polymer chemistry , genetics , enzyme
The preparation of 0.45 mm thin polyacrylamide gels, containing urea, for horizontal micro isoelectric focusing of milk proteins with PhastSystem is described. Isoelectric focusing in the small gels, stained either with Coomassie Brilliant Blue R‐250 or with the more sensitive silver stain, affords a fast and sensitive procedure for an analysis of milk and cheese proteins. The procedure can be effectively exploited in detecting adulteration in ovine cheese with bovine milk.