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A two‐dimensional electrophoresis procedure for single meristems of different forest species
Author(s) -
Bon MarieClaude
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100715
Subject(s) - meristem , botany , biology , isoelectric focusing , picea abies , pinus pinaster , cypress , biochemistry , shoot , enzyme
High resolution two‐dimensional electrophoresis, with isoelectric focusing in the first dimension and electrophoresis in sodium dodecyl sulfate in thin acrylamide gels in the second dimension, has been applied to separate the proteins of single meristems (200‐300 μm) from Sequoiadendrongiganteum, Sequoiasempervirens, Pseudotsuga menziesii, Picea abies, Pinus pinaster, Eucalyptus gunnii and Populus nigra . The technique may prove an efficient tool for studying inter‐ and intraclonal differences in meristem cultures of forest species, especially when referring to cloning from meristems of selected trees in relation to the rejuvenation process.