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Transparency of nitrocellulose membranes with triton X–114
Author(s) -
Vachereau Andre
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100713
Subject(s) - nitrocellulose , densitometer , transillumination , membrane , collodion , transparency (behavior) , chromatography , chemistry , materials science , analytical chemistry (journal) , optics , computer science , physics , biochemistry , computer security
Transparency of nitrocellulose membranes can be realized by simply impregnating the filters with concentrated Triton X‐114. Within a few seconds, protein blots become transparent and can be photographed by transillumination or scanned on a densitometer. Removal of the detergent is possible and the recovered filters can further be used for immunodetection purposes. This procedure is particularly well‐suited where extensive investigation of a single separated protein sample is required.