Transparency of nitrocellulose membranes with triton X–114 | Zendy

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Transparency of nitrocellulose membranes with triton X–114

Author(s) -

Vachereau Andre

Publication year - 1989

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150100713

Subject(s) - nitrocellulose , densitometer , transillumination , membrane , collodion , transparency (behavior) , chromatography , chemistry , materials science , analytical chemistry (journal) , optics , computer science , physics , biochemistry , computer security

Transparency of nitrocellulose membranes can be realized by simply impregnating the filters with concentrated Triton X‐114. Within a few seconds, protein blots become transparent and can be photographed by transillumination or scanned on a densitometer. Removal of the detergent is possible and the recovered filters can further be used for immunodetection purposes. This procedure is particularly well‐suited where extensive investigation of a single separated protein sample is required.

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