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Composition and genetic variability of proteins from nuclear fractions of mouse (DBA/2J and C57BL/6J) liver and brain
Author(s) -
Jungblut Peter,
ZimnyArndt Ursula,
Klose Joachim
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100704
Subject(s) - cytosol , nuclear protein , biology , genetic variation , nuclear membrane , cell nucleus , microbiology and biotechnology , membrane , gene , biochemistry , enzyme , transcription factor
Proteins from nuclear plasma of mouse liver and brain and from the nuclear membranes of mouse liver were separated by two‐dimensional electrophoresis. For the purpose of comparison, liver cytosol proteins were also investigated. The protein samples were prepared from two inbred strains of the mouse (DBA/2J, C57BL/6J) and their hybrids. The patterns obtained were compared with regard to the composition and genetic variability (qualitative and quantitative variants) of proteins from different nuclear fractions and organs. The percentage (> 30 %) of spots common to different organs (liver, brain), but from the same nuclear fraction (plasma) was greater than the percentage (< 20 %) of spots common to different cell and nuclear fractions (cytosol, nuclear plasma and nuclear membranes) of the same organ (liver). Quantitative genetic variants occurred much more frequently than qualitative genetic variants (5.1 % vs. 0.2 %; liver nuclear plasma). The incidence of genetic variants was much higher in liver (5.3 %) than in brains (0.0 %), and higher in solubilized nuclear proteins (5.3 %) than in structure‐bound nuclear proteins (2.1 %).