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The application of high resolution two‐dimensional polyacrylamide gel electrophoresis to the identification and purification of a protein, NG 8.4, present in Neisseria gonorrhoeae and the subsequent development of a radioimmunoassay
Author(s) -
Jackson Peter,
Urwin Valerie E.,
Torrance Michaela R.,
Carmen J. Anthony
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100703
Subject(s) - neisseria gonorrhoeae , polyacrylamide gel electrophoresis , neisseria , bacteria , chromatography , neisseriaceae , gel electrophoresis , biology , resolution (logic) , high resolution , microbiology and biotechnology , chemistry , biochemistry , enzyme , genetics , artificial intelligence , computer science , remote sensing , geology
A soluble protein of unknown function was shown, by two‐dimensional polyacrylamide gel electrophoresis, to be present in Neisseria gonorrhoeae at significantly higher concentrations than in the other related bacteria tested. The data indicate the possibility of this protein being specific to Neisseria gonorrhoeae . This protein was designated as NG 8.4 and purified. A radioimmunoassay was developed for the measurement of this protein and subsequently used to determine the degree of cross reaction exhibited by a number of species of bacteria. Of the bacteria tested only those of the genus Neisseria gave a significant reaction in the assay.