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Effect of nonparallel alternating fields on the mobility of DNA in the biased reptation model of gel electrophoresis
Author(s) -
Slater Gary W.,
Noolandi Jaan
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100520
Subject(s) - reptation , pulsed field gel electrophoresis , gel electrophoresis , electrophoresis , diagonal , field (mathematics) , gel electrophoresis of nucleic acids , intramolecular force , chemistry , chemical physics , physics , chromatography , polymer , nuclear magnetic resonance , mathematics , biochemistry , geometry , stereochemistry , genotype , pure mathematics , gene
Chromosome‐size DNA molecules can now be separated using a variety of pulsd field gel electrophoresis techniques. In this article, we study the predictions of the biased reptation model concerning the effect of two pulsed fields, making an arbicray angle, on the power of separation of gel electrophoresis. Separation is predicted to be largely enhanced for obtuse angles, in agreement with experiments. Interestingly very large molecules, which are not separated by pulsed fields, are predicted not to migrate along the gel diagonal for fairly long periods of time. Finally, we discuss the optimization of these techniques using the results of the theory, and the limitations of the latter when fluctuations and intramolecular modes probably dominate the system.