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Pulsed field gel electrophoresis: Studies of DNA migration made with the programmable, autonomously‐controlled electrode electrophoresis system
Author(s) -
Birren Bruce W.,
Hood Leroy,
Lai Eric
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100506
Subject(s) - electrophoresis , pulsed field gel electrophoresis , electric field , electrode , gel electrophoresis , dna , gel electrophoresis of nucleic acids , agarose gel electrophoresis , materials science , agarose , field (mathematics) , chromatography , analytical chemistry (journal) , chemistry , physics , biochemistry , mathematics , quantum mechanics , genotype , pure mathematics , gene
We have studied the migration of DNA in pulsed field agarose gels under a variety of electrophoresis conditions. We have made use of an instrument which can generate electric fields of any orientation, magnitude, or duration to compare different separation techniques for DNA molecules of from 1 to several thousand kilobase pairs. We discuss the capabilities of the system and present results of gel runs in which electrophoresis conditions were changed individually or in combination. The mobility of DNA in pulsed field gels is shown to reflect a number of interdependent physical parameters.