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Pulsed field electrophoresis in contour‐clamped homogeneous electric fields for the resolution of DNA by size or topology
Author(s) -
Chu Gilbert
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100504
Subject(s) - electric field , electrophoresis , topology (electrical circuits) , distortion (music) , homogeneous , molecule , field (mathematics) , resolution (logic) , dna , materials science , biological system , physics , chemistry , optoelectronics , computer science , chromatography , mathematics , artificial intelligence , electrical engineering , engineering , biology , statistical physics , quantum mechanics , amplifier , biochemistry , pure mathematics , cmos
The electrophoretic separation of DNA molecules can be controlled by the use of contour‐clamped homogeneous electric fields (CHEF). This paper describes an improved CHEF apparatus with negligible distortion in the electric fields. When the electric field was periodically reoriented, DNA molecules up to 2 megabases were resolved in a highly uniform manner. Furthermore, when the field strength was changed with orientation, topological variations of conventional‐sized DNA molecules were resolved.