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A simple method for accurately locating and cutting strips from the isoelectric focusing gel during two‐dimensional electrophoresis using the phastsystem
Author(s) -
Schiff Theodore A.,
Freedberg Irwin M.
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100313
Subject(s) - isoelectric focusing , strips , reproducibility , chromatography , simple (philosophy) , electrophoresis , sample (material) , computer science , biological system , artificial intelligence , chemistry , biology , biochemistry , philosophy , epistemology , enzyme
A rapid and simple method has been developed for precisely locating and cutting 1–3 mm strips from the isoelectric focusing gel during two‐dimensional electrophoresis using the PhastSystem. The protocol utilizes an easily constructed styrofoam template with marks to guide accurate cutting of the strips from the sample lanes. This system greatly enhances the reproducibility and quality of the two‐dimensional patterns obtained.