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Mirror densitometry for higher sensitivity and resolution
Author(s) -
Taube Dieter,
Neuhoff Volker
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100311
Subject(s) - densitometry , absorbance , resolution (logic) , sensitivity (control systems) , optics , reflection (computer programming) , materials science , chemistry , analytical chemistry (journal) , chromatography , physics , computer science , electronic engineering , artificial intelligence , programming language , engineering
In order to increase the pathway of the light inside a gel (or autoradiogram) during scanning, it is placed on top of a mirror and the densitometry is performed in a vertical reflection mode. As the light passes the gel a second time after being reflected by the mirror, the absorbance is nearly doubled. This increase in absorbance results in higher sensitivity and resolution.