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Program in BASIC for ferguson plot analysis, using a personal computer: Application to gel electrophoresis in a continuous buffer
Author(s) -
Gombocz Erich,
Chrambach Andreas
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100309
Subject(s) - plot (graphics) , computer science , function (biology) , curve fitting , personal computer , algorithm , computer graphics (images) , mathematics , computer hardware , statistics , machine learning , evolutionary biology , biology
A program in BASIC suitable for personal computers is described which is applicable to gel electrophoresis conducted in a single (continuous) buffer. The curve fitting is to a polynomial function, allowing for an objective selection of the most appropriate curve type and order – linear, convex or concave – in the particular application. Results do not differ significantly from previous programs for evaluation of linear Ferguson plots or of curve fitting to an exponential function for evaluating convex plots, executed on mainframe computers such as the DEC‐10 (Digital) and IBM 370 computers. Thus, the program combines original versatility with, for the first time, the possibility for widespread application of Ferguson plot analysis on personal computers.