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A simple, sensitive method of analyzing bacterial ribosomal DNA polymorphism
Author(s) -
PicardPasquier Nicole,
Ouagued Malika,
Picard Bertrand,
Goullet Philippe,
Krishnamoorthy Rajagopal
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100306
Subject(s) - ribosomal rna , dna , biology , primer (cosmetics) , bacteria , ribosomal dna , genetics , reverse transcriptase , dna extraction , computational biology , rna , microbiology and biotechnology , polymerase chain reaction , gene , chemistry , phylogenetics , organic chemistry
A rapid DNA extraction procedure and random primer labelling of Fscherichia coli ribosomal RNA with cloned reverse transcriptase have been used to establish a simple and highly sensitive method for studying ribosomal DNA polymorphism in bacteria. Examples of inter‐ and intraspecies differentiation of bacteria are given and potential applications in bacterial epidemiology and taxonomy are discussed.