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Fully automated capillary isotachophoresis of proteins
Author(s) -
Manabe Takashi,
Yamamoto Hideko,
Okuyama Tsuneo
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100303
Subject(s) - isotachophoresis , chromatography , capillary action , electrolyte , microcomputer , analytical chemistry (journal) , chemistry , computer science , materials science , telecommunications , chip , electrode , composite material
An apparatus for fully automated capillary isotachophoresis was constructed. A commercial apparatus (Shimadzu IP‐2A) was modified in the electrolyte pumping system and the flow lines were simplified. An automatic sampler was used for sequential sampling. The equipment, pumps, the sampler, a high‐voltage DC power supply, and a recorder, were controlled by a system controller which comprises a microcomputer and a BASIC program for time‐control of the equipments. The apparatus was successfully used for the automated sequential analysis of human serum proteins. Forty serum samples were analyzed within 17 h without manual operation and for each sample the serum proteins were resolved into about twenty UV peaks or shoulders.