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Effect of electric field switching on the electrophoretic mobility of single‐stranded DNA molecules in polyacrylamide gels
Author(s) -
Lai Eric,
Davi Noelle A.,
Hood Leroy E.
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100116
Subject(s) - electrophoresis , electric field , dna , gel electrophoresis of nucleic acids , polyacrylamide , molecule , pulsed field gel electrophoresis , gel electrophoresis , polyacrylamide gel electrophoresis , chemistry , single molecule real time sequencing , materials science , chromatography , biophysics , analytical chemistry (journal) , dna sequencing , dna sequencer , biochemistry , biology , polymer chemistry , physics , organic chemistry , quantum mechanics , genotype , gene , enzyme
We have examined the effects of pulsed electric fields on the separation of single‐stranded DNA molecules in polyacrylamide sequencing gels. Using different electric field pulsing regimens, the mobilities of single‐stranded DNA molecules can be retarded or increased as compared to conventional electrophoresis. These results indicated that pulsed field techniques can be applied to gel electrophoresis of small single‐stranded DNA molecules.