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Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis without a stacking gel: Use for high sample throughput
Author(s) -
Marshall Thomas,
Gaston S. J. Stephen,
Williams Katherine M.
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100115
Subject(s) - agarose , polyacrylamide , polyacrylamide gel electrophoresis , chromatography , sodium dodecyl sulfate , stacking , gel electrophoresis , molecular weight size marker , chemistry , gel electrophoresis of nucleic acids , electrophoresis , gel electrophoresis of proteins , resolution (logic) , biochemistry , computer science , organic chemistry , polymer chemistry , enzyme , artificial intelligence
Sodium dodecyl sulfate‐polyacrylamide gel electrophoresis without a stacking gel minimizes lateral spreading of protein when samples are applied in agarose wells and allows high sample throughput (6 samples/cm gel width). The method is simple and convenient to use and gives comparable resolution to the standard method with 4–20% or 6–30% polyacrylamide gradient gels. Best results are obtained when the upper zone of the separating gel is of low polyacrylamide concentration. This indicates a need for the molten agarose to penetrate and anneal with the separating gel.