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Identification of different classes of superoxide dismutase in carnation petals
Author(s) -
Droillard MarieJo,
Bureau Danielle,
Paulin André,
Daussant Jean
Publication year - 1989
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150100111
Subject(s) - carnation , superoxide dismutase , isoelectric focusing , petal , isozyme , chemistry , biochemistry , enzyme , electrophoresis , chromatography , biology , botany
Isoelectric focusing and nondenaturing two‐dimensional electrophoresis, followed by an enzymatic characterization involving use of specific inhibitors were applied to identify the different forms of the superoxide dismutase (SOD) extracted from petals of cut carnation. Electrophoresis was carried out either with the crude extract or with fractions obtained after ion exchange chromatography of the extract. Three forms of the SOD were identified (Cu, Zn‐SOD, Fe‐SOD and Mn‐SOD). They have close pI 's (4.7 to 4.9) and most of them are composed of several isoforms.