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Improvement of electrophoretic transfer by casting acrylamide gels on a cellophane sheet
Author(s) -
Abbal Michel,
Julie Claudine
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150091211
Subject(s) - cellophane , acrylamide , casting , electrophoresis , chemistry , materials science , chromatography , polymer science , composite material , polymer , copolymer
Electrophoretic transfer of protein after isolectric focusing using a polyacrylamide gel of less than 0.5 mm is difficult if the gel is backed to an electrically nonconductive casting support, such as glass plate or plastic films. By casting the gel on a cellophane sheet, it is not necessary to remove the gel from the support prior to electrophoretic transfer. The use of a cellophane support does not alter the quality of the final pattern.