Extraction of plant proteins for two‐dimensional electrophoresis | Zendy

Granier Fabienne | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Extraction of plant proteins for two‐dimensional electrophoresis

Author(s) -

Granier Fabienne

Publication year - 1988

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150091106

Subject(s) - trichloroacetic acid , chromatography , chemistry , extraction (chemistry) , sodium dodecyl sulfate , electrophoresis , acetone , tris , protein purification , gel electrophoresis , membrane protein , solubilization , biochemistry , membrane

Three different extraction procedures for two‐dimensional electrophoresis of plant proteins are compared: (i) extraction of soluble proteins with a nondenaturing Trisbuffer, (ii) denaturing extraction in presence of sodium dodecyl sulfate at elevated temperature allowing the solubilization of membrane proteins in addition to a recovery of soluble proteins, and (iii) a trichloroacetic acid‐acetone procedure allowing the direct precipitation of total proteins.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research