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Extraction of plant proteins for two‐dimensional electrophoresis
Author(s) -
Granier Fabienne
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150091106
Subject(s) - trichloroacetic acid , chromatography , chemistry , extraction (chemistry) , sodium dodecyl sulfate , electrophoresis , acetone , tris , protein purification , gel electrophoresis , membrane protein , solubilization , biochemistry , membrane
Three different extraction procedures for two‐dimensional electrophoresis of plant proteins are compared: (i) extraction of soluble proteins with a nondenaturing Trisbuffer, (ii) denaturing extraction in presence of sodium dodecyl sulfate at elevated temperature allowing the solubilization of membrane proteins in addition to a recovery of soluble proteins, and (iii) a trichloroacetic acid‐acetone procedure allowing the direct precipitation of total proteins.