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Identification of group specific component/vitamin D‐binding protein (GC/DBP) mutants by isoelectric focusing in immobilized pH gradients
Author(s) -
Constans Jacques,
Cleve Hartwig
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090922
Subject(s) - isoelectric focusing , vitamin d binding protein , isoelectric point , chemistry , chromatography , immobilized ph gradient , electrophoresis , mutant , acrylamide , biochemistry , vitamin , gene , monomer , enzyme , organic chemistry , polymer
The six common genetic types of the group specific component/vitamin D‐binding protein (GC/DBP) system are usually classified by isoelectric focusing in carrier ampholytes, followed by visualization of the GC proteins by immunoprinting with monospecific antiserum. In addition, more than 120 mutant GC types have been discovered. For their identification additional methods were necessary, including poly acrylamide gel electrophoresis, isoelectric focusing in the presence of 3 M urea as well as isoelectric focusing in immobilized pH gradients. The application of the last method is described in detail and several examples of GC/DBP mutants identified thereby are presented.