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Microheterogeneity of human plasma lecithin:cholesterol acyltransferase examined by isoelectric focusing in immobilized pH gradients
Author(s) -
Holmquist Leif,
Bjellqvist Bengt
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090919
Subject(s) - isoelectric focusing , lecithin , lecithin—cholesterol acyltransferase , chromatography , chemistry , human plasma , cholesterol , sterol o acyltransferase , biochemistry , isoelectric point , plasma , enzyme , lipoprotein , physics , quantum mechanics
The microheterogeneity of highly purified human plasma lecithin:cholesterol acyltransferase (LCAT) has been examined by electrophoresis in immobilized pH gradients in Immobiline‐polyacrylamide gels of the pH ranges 4–7 and 4.2–4.9. Seven isoforms were obtained with LCAT isolated from pools of normal plasma. Using this technique the apparent p I values at 15 °C for the isoforms in the pH 4.2–4.9 gradient were 4.37, 4.42, 4.48, 4.53, 4.60, 4.67 and 4.74. (SD = ± 0.03 for all). The most intensely stained band in the isoform pattern corresponded to the isoform with a p I value of 4.48.