Isoelectric focusing of apolipoproteins in immobilized pH gradients: Improved determination of apolipoprotein Ephenotypes | Zendy

Baumstrak Manfred W. | Zendy; Berg Aloys | Zendy; Halle Martin | Zendy; Keul Joseph | Zendy

Premium

Isoelectric focusing of apolipoproteins in immobilized pH gradients: Improved determination of apolipoprotein Ephenotypes

Author(s) -

Baumstrak Manfred W.,

Berg Aloys,

Halle Martin,

Keul Joseph

Publication year - 1988

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.1150090918

Subject(s) - isoelectric focusing , apolipoprotein b , chemistry , gene isoform , chromatography , isoelectric point , immobilized ph gradient , very low density lipoprotein , biochemistry , lipoprotein , cholesterol , enzyme , gene

A method for isoelectric focusing of apolipoprotein Ein an immobilized pH gradient with added carrier ampholytes has been developed. This method is an improvement over conventional isoelectric focusing of apolipoprotein E with respect to resolution reproducibility, and simplicity. Since monosialo isoforms are resolved from the not mally cofocusing asialo isoforms, unique patterns are obtained for all 6 common apolipoprotein E phenotypes. The method can also be applied to the screening of apolipoprotein A and C isoforms. Delipidated very low density lipoproteins (VLDL) have been used as the source of apolipoprotein E and C. Apolipoprotein A isoforms were focused directly from detergent‐treated serum. Immunodetection of apolipo protein E using capillary transfer was found to be compatible with the described method.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research