Premium
Isoelectric focusing of apolipoproteins in immobilized pH gradients: Improved determination of apolipoprotein Ephenotypes
Author(s) -
Baumstrak Manfred W.,
Berg Aloys,
Halle Martin,
Keul Joseph
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090918
Subject(s) - isoelectric focusing , apolipoprotein b , chemistry , gene isoform , chromatography , isoelectric point , immobilized ph gradient , very low density lipoprotein , biochemistry , lipoprotein , cholesterol , enzyme , gene
A method for isoelectric focusing of apolipoprotein Ein an immobilized pH gradient with added carrier ampholytes has been developed. This method is an improvement over conventional isoelectric focusing of apolipoprotein E with respect to resolution reproducibility, and simplicity. Since monosialo isoforms are resolved from the not mally cofocusing asialo isoforms, unique patterns are obtained for all 6 common apolipoprotein E phenotypes. The method can also be applied to the screening of apolipoprotein A and C isoforms. Delipidated very low density lipoproteins (VLDL) have been used as the source of apolipoprotein E and C. Apolipoprotein A isoforms were focused directly from detergent‐treated serum. Immunodetection of apolipo protein E using capillary transfer was found to be compatible with the described method.