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Electroblotting of Immobiline DryPlates applied to identification of human plasma apolipoprotein A‐I
Author(s) -
Holmquist Leif
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090910
Subject(s) - electroblotting , nitrocellulose , chromatography , agarose , polyacrylamide , polyacrylamide gel electrophoresis , chemistry , membrane , gel electrophoresis , apolipoprotein b , sodium dodecyl sulfate , biochemistry , polymer chemistry , enzyme , cholesterol
A method for efficient electroblotting of Immobiline DryPlates, allowing subsequent immunological identification of separated proteins has been developed. A thin layer of 1 % agarose containing sodium dodecyl sulfate is moulded on the 0.5 mm thick polyacrylamide gel surface after completed electrophoresis. After separation of the agarose‐polyacrylamide gel sandwich from the plastic film the rigid gel sandwich could be easily transferred to a nitrocellulose membrane and electroblotting could be performed without adherence of the sticky polyacrylamide gel layer to the membrane. Using this technique human plasma high density apolipoprotein A‐Iisoforms, over a wide concentration range, could be identified in a heterogeneous mixture, conserving the isoform pattern and band sharpness produced in the immobilized pH gradient experiments.