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A new sample applicator for isoelectric focusing in horizontal polyacrylamide gels
Author(s) -
Altland Klaus,
Becher Pia
Publication year - 1988
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.1150090907
Subject(s) - isoelectric focusing , capillary action , electrophoresis , sample (material) , materials science , chromatography , flat glass , polyacrylamide , optics , analytical chemistry (journal) , chemistry , composite material , polymer chemistry , physics , biochemistry , enzyme
A new sample applicator for horizontal flat gels is described. The applicator is practically safe against contamination from adjacent samples and can be used for all types of electrophoretic separations including a concentration step for either the sample ( i. e. disc electrophoresis) or the separated zones ( i. e. isoelectric focusing). The applicator is a piece of flat glass with 26 or 51 parallel 2 mm wide grooves, drilled at distances of 9 or 4.5 mm. Samples, maximally 25 or 50. are applied to the areas between the grooves. By inverting the applicator, the samples are brought into close vicinity to the gel surface and the pendant droplets expand by capillary attraction into the slits between the glass and gel with resultant even distribution across the lanes of 2.5 or 7 mm width. The applicator can be used for separations with and without protection of the electrophoretic setup by paraffin oil and allows for fast multiple handling of samples by means of appropriate syringes and microtiter plates.